NEW ASSAYS FOR TRANSKETOLASE

被引：28

作者：

HECQUET, L

BOLTE, J

DEMUYNCK, C

机构：

[1] Université Blaise Pascal, Laboratoire de Chimie Organique Biologique, URA 485 du CNRS

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 12期

关键词：

D O I：

10.1271/bbb.57.2174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Given the importance of transketolase, TK (EC 2.2.1.1) for both pharmacological studies and synthetic purposes, the need for a simple and inexpensive assay is patent. We describe here a simple and inexpensive TK assay using the unphosphorylated ketoses L-erythrulose, 4-deoxy-L-erythrulose, and 4-deoXy-D,L-erythrulose as donor substrates instead Of D-Xylulose-5-phosphate, with D-ribose-5-phosphate as the acceptor substrate.

引用

页码：2174 / 2176

页数：3

共 14 条

[1]

BERGMEYER HU, 1974, METHOD ENZYMAT AN, P1446

[2] UTILIZATION OF ENZYMES IN ORGANIC-CHEMISTRY - TRANSKETOLASE CATALYZED SYNTHESIS OF KETOSES [J].