THE LACTATE/PYRUVATE SHUTTLE IN SPERMATOZOA - OPERATION IN-VITRO

被引：39

作者：

GALLINA, FG ^{[1
]}

DEBURGOS, NMG ^{[1
]}

BURGOS, C ^{[1
]}

CORONEL, CE ^{[1
]}

BLANCO, A ^{[1
]}

机构：

[1] NATL UNIV CORDOBA,FAC CIENCIAS MED,CATEDRA QUIM BIOL,RA-5016 CORDOBA,ARGENTINA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 308卷 / 02期

关键词：

D O I：

10.1006/abbi.1994.1072

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The operation of a shuttle for the transfer of reducing equivalents in the special mitochondria present in the middle piece of spermatozoa (sperm-type mitochondria, STM) was studied in reconstituted systems in vitro with mouse, rat, and rabbit STM. The redox couple lactate/ pyruvate and the lactate dehydrogenase isozyme C-4 are involved in the shuttle. It is active with rat and rabbit STM, while it does not work with mouse STM, probably because the influx of lactate into the mouse organelles is relatively poor. Ratios of consumption of pyruvate/lactate by STM were 21.6, 1.28, and 1.6 for mouse, rat, and rabbit organelles, respectively. The shuttle is inhibited by O.6 mM mersalyl, a blocker of lactate transport. The operation of the shuttle would oxidize cytosolic NADH produced during aerobic glycolysis (or fructolysis) in spermatozoa of those species having an efficient lactate carrier in mitochondria. (C) 1994 Academic Press,Inc.

引用

页码：515 / 519

页数：5

共 21 条

[1] BERGMEYER HU, 1974, METHOD ENZYMAT AN, V3, P1446
[2] PROPERTIES OF TESTICULAR LACTATE-DEHYDROGENASE ISOENZYME
BLANCO, A
BURGOS, C
GEREZDEBURGOS, NM
MONTAMAT, EE
[J]. BIOCHEMICAL JOURNAL, 1976, 153 (02) : 165 - 172
[3] BREMER J, 1975, BIOCHIM BIOPHYS ACTA, V76, P387
[4] STUDIES INVITRO ON SHUTTLE SYSTEMS OF MOUSE SPERMATOZOA
BURGOS, C
CORONEL, CE
DEBURGOS, NM
ROVAI, LE
BLANCO, A
[J]. BIOCHEMICAL JOURNAL, 1982, 208 (02) : 413 - 417
[5] MITOCHONDRIAL TRANSPORT PROCESSES AND OXIDATION OF NADH BY HYPOTONICALLY-TREATED BOAR SPERMATOZOA
CALVIN, J
TUBBS, PK
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 89 (01): : 315 - 320
[6] CHANCE B, 1956, ADV ENZYMOL REL S BI, V17, P65
[7] PROPERTIES OF THE BRANCHED-CHAIN 2-HYDROXY ACID 2-OXO ACID SHUTTLE IN MOUSE SPERMATOZOA
CORONEL, CE
GALLINA, FG
DEBURGOS, NMG
BURGOS, C
BLANCO, A
[J]. BIOCHEMICAL JOURNAL, 1986, 235 (03) : 853 - 858
[8] CATALYTIC PROPERTIES OF THE SPERM-SPECIFIC LACTATE-DEHYDROGENASE (LDH-X OR C-4) FROM DIFFERENT SPECIES
CORONEL, CE
BURGOS, C
DEBURGOS, NMG
ROVAI, LE
BLANCO, A
[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY, 1983, 225 (03): : 379 - 385
[9] DEBURGOS NMG, 1978, BIOCHEM BIOPH RES CO, V81, P644
[10] EFFECT OF L-MALATE ON PYRUVATE-DEHYDROGENASE ACTIVITY OF SPERMATOZOA
DEBURGOS, NMG
GALLINA, F
BURGOS, C
BLANCO, A
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 308 (02) : 520 - 524

← 1 2 3 →