EFFECT OF L-MALATE ON PYRUVATE-DEHYDROGENASE ACTIVITY OF SPERMATOZOA

被引：24

作者：

DEBURGOS, NMG ^{[1
]}

GALLINA, F ^{[1
]}

BURGOS, C ^{[1
]}

BLANCO, A ^{[1
]}

机构：

[1] NATL UNIV CORDOBA,FAC CIENCIAS MED,CATEDRA QUIM BIOL,RA-5016 CORDOBA,ARGENTINA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 308卷 / 02期

关键词：

D O I：

10.1006/abbi.1994.1073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Presence of L-malate (from 0.5 to 4 mM concentrations) in the medium produces a marked increment of pyruvate consumption by the special type of mitochondria found in the middle piece of spermatozoa (sperm-type mitochondria, STM). Pyruvate uptake by liver mitochondria is not increased by malate. A comparative study on pyruvate dehydrogenase (PDH) of STM and liver mitochondria from mouse, rat, and rabbit showed that 2 mM L-malate does not modify significantly the activity of liver PDH, while it increases markedly that of spermatozoal PDH in the three species. The differential sensitivity to L-malate appears to be a peculiar regulatory property of the PDH complex in the gametes, which contains at least one component (E1, pyruvate decarboxylase, EC 1.2.4.1) known to be a sperm-specific isozyme. (C) 1994 Academic Press, Inc.

引用

页码：520 / 524

页数：5

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