INHIBITION OF PHOSPHOLIPASE-A2 PURIFIED FROM HUMAN HERNIATED DISK

被引：12

作者：

CARABAZA, A ^{[1
]}

CABRE, F ^{[1
]}

GARCIA, AM ^{[1
]}

GOMEZ, M ^{[1
]}

SANCHEZ, J ^{[1
]}

MAULEON, D ^{[1
]}

CARGANICO, G ^{[1
]}

机构：

[1] LABS MENARINI SA, DEPT R&D, ALFONSO XII 587, E-08912 BADALONA, SPAIN

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 45卷 / 03期

关键词：

D O I：

10.1016/0006-2952(93)90158-S

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect on human herniated intervertebral disc phospholipase A2 (HD-PLA2) of a number of retinoids, antirheumatic drugs and reported PLA2 inhibitors was evaluated using autoclaved [1-C-14]oleate-labeled Escherichia coli membranes as the substrate. Dexamethasone, non-steroidal antiinflammatory drugs, aristolochic acid and retinol were inactive, whereas a marked inhibition was found for manoalide, retinal, nordihydroguaiaretic acid and p-bromophenacyl bromide after preincubation with the enzyme (IC50 values 0.25, 4, 5 and 5 muM, respectively). The results are parallel to those obtained with the PLA2 purified from human synovial fluid.

引用

页码：783 / 786

页数：4

共 27 条

[21]

ROBERTS MF, 1977, J BIOL CHEM, V252, P2405

[22] EFFECTS OF ARISTOLOCHIC ACID ON PHOSPHOLIPASE-A2 ACTIVITY AND ARACHIDONATE METABOLISM OF HUMAN-NEUTROPHILS [J].

ROSENTHAL, MD ;

VISHWANATH, BS ;

FRANSON, RC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1001 (01) :1-8

[23] HIGH-LEVELS OF INFLAMMATORY PHOSPHOLIPASE-A2 ACTIVITY IN LUMBAR-DISK HERNIATIONS [J].

SAAL, JS ;

FRANSON, RC ;

DOBROW, R ;

SAAL, JA ;

WHITE, AH ;

GOLDTHWAITE, N .

SPINE, 1990, 15 (07) :674-678

[24] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[25]

VADAS P, 1988, ARTHRITIS RHEUM, V31, pS31

[26] EDEMA-INDUCING ACTIVITY OF PHOSPHOLIPASE-A2 PURIFIED FROM HUMAN SYNOVIAL-FLUID AND INHIBITION BY ARISTOLOCHIC ACID [J].

VISHWANATH, BS ;

FAWZY, AA ;

FRANSON, RC .

INFLAMMATION, 1988, 12 (06) :549-561

[27] HISTIDINE AT ACTIVE-SITE OF PHOSPHOLIPASE-A2 [J].

VOLWERK, JJ ;

PIETERSON, WA ;

HAAS, GHD .

BIOCHEMISTRY, 1974, 13 (07) :1446-1454

← 1 2 3 →