LINEAR-DICHROISM SPECTROSCOPY FOR THE STUDY OF STRUCTURAL-PROPERTIES OF PROTEINS

被引：9

作者：

BLOEMENDAL, M ^{[1
]}

VANGRONDELLE, R ^{[1
]}

机构：

[1] FREE UNIV AMSTERDAM, DEPT BIOPHYS, 1081 HV AMSTERDAM, NETHERLANDS

来源：

MOLECULAR BIOLOGY REPORTS | 1993年 / 18卷 / 01期

关键词：

LINEAR DICHROISM; MOLECULAR ORIENTATION; PHOTOSELECTION; POLARIZED SPECTROSCOPY; PROTEIN-DNA INTERACTION; PROTEIN STRUCTURE;

D O I：

10.1007/BF01006895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review gives an experiment directed survey of the application of linear-dichroism (LD) spectroscopy to the study of proteins. LD spectroscopy is a relatively simple technique that provides information on the orientation of chromophores in molecules, on molecular characteristics such as shape, size and electronic properties, and on binding parameters in molecular complexes. Since LD is only observed when the molecules are non-randomly oriented in the sample, particular attention is paid to various orientation techniques, viz. in electric and flow fields, in polymer films and gels, and by light induction (photoselection). Examples are given on bacteriorhodopsin and retinals, chlorosomes, lens crystallins, aspartate aminotransferase, and the interaction of gene32- and recA-protein with DNA.

引用

页码：49 / 69

页数：21

共 141 条

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