RABBIT SPERM FUNCTION AFTER COCULTURE WITH UTERINE EPITHELIAL-CELLS

被引:4
作者
BOICE, ML [1 ]
DUBY, RT [1 ]
ROBL, JM [1 ]
机构
[1] UNIV MASSACHUSETTS, DEPT VET & ANIM SCI, 304 PAIGE LAB, AMHERST, MA 01003 USA
关键词
co-culture; sperm function; uterine epithelium;
D O I
10.1016/0093-691X(90)90582-E
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effects of spermatozoa:uterine epithelial cell interactions in vitro on various sperm functions were studied using monolayers of uterine epithelial cells, endometrial stromal cells and fetal fibroblasts. Epithelial and stromal cells were isolated from uteri of rabbits in estrus, while fibroblasts were derived from 12-d-old rabbit fetuses. Twenty-nine to 31 h after culture initiation, washed, ejaculated rabbit spermatozoa were incubated with epithelial cells, uterine stromal or fetal fibroblastic cells, medium or conditioned medium. Sperm viability and loss of acrosome were measured after 10 to 20 h of incubation. Progressive sperm motility and fertilizing ability, which was assessed by an in vivo fertilization assay, were determined after 12 h co-culture. Sperm viability decreased throughout the culture period and was not affected by treatment. Sperm co-cultured with epithelial cells or incubated in medium had fewer acrosomes after 20 h than after 10 h. Fewer sperm co-cultured with stromal or fibroblastic cells lost their acrosomes. Progressive motility was positively affected by sperm-epithelium interaction as 39% of the co-cultured sperm were motile compared with 18% of the sperm incubated in media. In vivo fertilization experiments suggested that sperm incubated with epithelial cells or in medium had similar fertilizing ability. The co-culture of sperm with uterine cells provides an in vitro model to evaluate the effect of gamete-genital tract interaction on sperm function. © 1990.
引用
收藏
页码:113 / 125
页数:13
相关论文
共 36 条
[31]  
Steel R. G. D., 1980, Principles and procedures of statistics, a biometrical approach.
[32]   SPERM TRANSPORT AND MOTILITY IN THE MOUSE OVIDUCT - OBSERVATIONS INSITU [J].
SUAREZ, SS .
BIOLOGY OF REPRODUCTION, 1987, 36 (01) :203-210
[33]  
VANBLERKOM J, 1979, CELLULAR BASIS MAMMA, P135
[34]   SPEED OF SPERMATOZOAN TRANSPORT IN REPRODUCTIVE TRACT OF ESTROUS COW [J].
VANDEMARK, NL ;
MOELLER, AN .
AMERICAN JOURNAL OF PHYSIOLOGY, 1951, 165 (03) :674-679
[35]   THE FERTILIZATION PERFORMANCE INVIVO OF RABBIT SPERMATOZOA CAPACITATED INVITRO [J].
VIRIYAPANICH, P ;
BEDFORD, JM .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1981, 216 (01) :169-174
[36]  
Yanagimachi R, 1981, FERTILIZATION EMBRYO, P81