IDENTIFICATION OF A SEQUENCE IN THE PEPCK GENE THAT MEDIATES A NEGATIVE EFFECT OF INSULIN ON TRANSCRIPTION

被引：341

作者：

OBRIEN, RM ^{[1
]}

LUCAS, PC ^{[1
]}

FOREST, CD ^{[1
]}

MAGNUSON, MA ^{[1
]}

GRANNER, DK ^{[1
]}

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MOLEC PHYSIOL & BIOPHYS,NASHVILLE,TN 37232

来源：

SCIENCE | 1990年 / 249卷 / 4968期

关键词：

D O I：

10.1126/science.2166335

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phosphoenolpyruvate carboxykinase (PEPCK) governs the rate-limiting step in gluconeogenesis. Glucocorticoids and adenosine 3′,5′-monophosphate (cAMP) increase PEPCK gene transcription and gluconeogenesis, whereas insulin has the opposite effect. Insulin is dominant, since it prevents cAMP and glucocorticoid-stimulated transcription. Glucocorticoid and cAMP response elements have been located in the PEPCK gene and now a 15-base pair insulin-responsive sequence (IRS) is described. Evidence for a binding activity that recognizes this sequence is presented.

引用

页码：533 / 537

页数：5

共 35 条

[1]

ALEXANDER M, 1985, J BIOL CHEM, V260, P1978

[2]

ANDREONE TL, 1982, J BIOL CHEM, V257, P35

[3]

CHU DTW, 1988, J BIOL CHEM, V263, P13007

[4] REGULATION OF GENE-EXPRESSION BY INSULIN IN ADIPOSE-CELLS - OPPOSITE EFFECTS ON ADIPSIN AND GLYCEROPHOSPHATE DEHYDROGENASE GENES [J].

DANI, C ;

BERTRAND, B ;

BARDON, S ;

DOGLIO, A ;

AMRI, E ;

GRIMALDI, P .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1989, 63 (1-2) :199-208

[5]

DENTON RM, 1986, ADV CYCLIC NUCL PROT, V20, P293

[6]

DRANGINIS A, 1984, J BIOL CHEM, V259, P2216

[7] SEVERELY IMPAIRED ADIPSIN EXPRESSION IN GENETIC AND ACQUIRED OBESITY [J].

FLIER, JS ;

COOK, KS ;

USHER, P ;

SPIEGELMAN, BM .

SCIENCE, 1987, 237 (4813) :405-408

[8] TISSUE-SPECIFIC INVITRO TRANSCRIPTION FROM THE MOUSE ALBUMIN PROMOTER [J].

GORSKI, K ;

CARNEIRO, M ;

SCHIBLER, U .

CELL, 1986, 47 (05) :767-776

[9] INHIBITION OF TRANSCRIPTION OF THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE BY INSULIN [J].

GRANNER, D ;

ANDREONE, T ;

SASAKI, K ;

BEALE, E .

NATURE, 1983, 305 (5934) :549-551

[10] EFFECTS OF CYCLIC ADENOSINE-MONOPHOSPHATE, DEXAMETHASONE AND INSULIN ON PHOSPHOENOLPYRUVATE CARBOXYKINASE SYNTHESIS IN REUBER H-35 HEPATOMA-CELLS [J].

GUNN, JM ;

TILGHMAN, SM ;

HANSON, RW ;

RESHEF, L ;

BALLARD, FJ .

BIOCHEMISTRY, 1975, 14 (11) :2350-2357

← 1 2 3 4 →