IDENTIFICATION OF A SEQUENCE IN THE PEPCK GENE THAT MEDIATES A NEGATIVE EFFECT OF INSULIN ON TRANSCRIPTION

被引：341

作者：

OBRIEN, RM ^{[1
]}

LUCAS, PC ^{[1
]}

FOREST, CD ^{[1
]}

MAGNUSON, MA ^{[1
]}

GRANNER, DK ^{[1
]}

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MOLEC PHYSIOL & BIOPHYS,NASHVILLE,TN 37232

来源：

SCIENCE | 1990年 / 249卷 / 4968期

关键词：

D O I：

10.1126/science.2166335

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phosphoenolpyruvate carboxykinase (PEPCK) governs the rate-limiting step in gluconeogenesis. Glucocorticoids and adenosine 3′,5′-monophosphate (cAMP) increase PEPCK gene transcription and gluconeogenesis, whereas insulin has the opposite effect. Insulin is dominant, since it prevents cAMP and glucocorticoid-stimulated transcription. Glucocorticoid and cAMP response elements have been located in the PEPCK gene and now a 15-base pair insulin-responsive sequence (IRS) is described. Evidence for a binding activity that recognizes this sequence is presented.

引用

页码：533 / 537

页数：5

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