ATP DEPENDENT HISTONE PHOSPHORYLATION AND NUCLEOSOME ASSEMBLY IN A HUMAN CELL-FREE-EXTRACT

被引：17

作者：

BANERJEE, S ^{[1
]}

BENNION, GR ^{[1
]}

GOLDBERG, MW ^{[1
]}

ALLEN, TD ^{[1
]}

机构：

[1] CRC PATERSON INST CANC RES,MANCHESTER M20 9BX,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 21期

关键词：

D O I：

10.1093/nar/19.21.5999

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Physiologically spaced nucleosome formation in HeLa cell extracts is ATP dependent. ATP hydrolysis is required for chromatin assembly on both linear and covalently closed circular DNA. The link between the phosphorylation state of histones and nucleosome formation has been examined and we demonstrate that in the absence of histone phosphorylation no stable and regularly spaced nucleosomes are formed. Phosphorylated H3 stabilizes the nucleosome core; while phosphorylation of histone H2a is necessary to increase the linker length between nucleosomes from 0 to approximately 45 bp. Histone H1 alone, whether phosphorylated or unphosphorylated, does not increase the nucleosome repeat length in the absence of core histone phosphorylation. Phosphorylations of H1 and H3 correlate with condensation of chromatin. Maximum ATP hydrolysis which is necessary to increase the periodicity of nucleosomes from approximately 150 to approximately 185 bp, not only inhibits H1 and H3 phosphorylation but facilitates their dephosphorylation.

引用

页码：5999 / 6006

页数：8

共 54 条

[11] DEPHOSPHORYLATION AND ACTIVATION OF XENOPUS-P34CDC2 PROTEIN-KINASE DURING THE CELL-CYCLE [J].

GAUTIER, J ;

MATSUKAWA, T ;

NURSE, P ;

MALLER, J .

NATURE, 1989, 339 (6226) :626-629

[12] FOLDING OF DNA DOUBLE HELIX IN CHROMATIN-LIKE STRUCTURES FROM SIMIAN VIRUS 40 [J].

GERMOND, JE ;

HIRT, B ;

OUDET, P ;

GROSSBELLARD, M ;

CHAMBON, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) :1843-1847

[13]

GILKIN GC, 1984, CELL, V37, P33

[14] DIFFERENT NUCLEOSOME SPACING IN TRANSCRIBED AND NON-TRANSCRIBED REGIONS OF THE RIBOSOMAL-RNA GENE IN TETRAHYMENA-THERMOPHILA [J].

GOTTSCHLING, DE ;

PALEN, TE ;

CECH, TR .

NUCLEIC ACIDS RESEARCH, 1983, 11 (07) :2093-2109

[15]

GRAZIANI Y, 1983, J BIOL CHEM, V258, P6344

[16] INTERACTION OF SPERM HISTONE VARIANTS AND LINKER DNA DURING SPERMIOGENESIS IN THE SEA-URCHIN [J].

GREEN, GR ;

POCCIA, DL .

BIOCHEMISTRY, 1988, 27 (02) :619-625

[17] CELL CYCLE-SPECIFIC CHANGES IN HISTONE PHOSPHORYLATION ASSOCIATED WITH CELL-PROLIFERATION AND CHROMOSOME CONDENSATION [J].

GURLEY, LR ;

WALTERS, RA ;

TOBEY, RA .

JOURNAL OF CELL BIOLOGY, 1974, 60 (02) :356-364

[18] HISTONE PHOSPHORYLATION AND CHROMATIN STRUCTURE DURING MITOSIS IN CHINESE-HAMSTER CELLS [J].

GURLEY, LR ;

DANNA, JA ;

BARHAM, SS ;

DEAVEN, LL ;

TOBEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 84 (01) :1-15

[19] PHOSPHORYLATION AT CLUSTERED SER-PRO-X-LYS ARG MOTIFS IN SPERM-SPECIFIC HISTONE-H1 AND HISTONE-H2B [J].

HILL, CS ;

PACKMAN, LC ;

THOMAS, JO .

EMBO JOURNAL, 1990, 9 (03) :805-813

[20] CONTROL OF CHROMOSOME CONDENSATION IN THE SEA-URCHIN EGG [J].

KRYSTAL, GW ;

POCCIA, D .

EXPERIMENTAL CELL RESEARCH, 1979, 123 (02) :207-219

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