HIGH-YIELD EXPRESSION, REFOLDING, AND PURIFICATION OF PENICILLIN-BINDING PROTEIN 2A FROM METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS STRAIN 27R

The mecA-27R gene, which encodes PBP2a from methicillin-resistant Staphylococcus,; aureus strain 27R, was modified to remove the putative N-terminal membrane-spanning region, cloned into the T7 RNA polymerase expression vector pET11d, and used to transform Escherichia coli strain BL21(DE3). The majority of PBP2a was expressed in the form of inclusion bodies, which were extracted, denatured, and refolded, The protein was then purified by anion-exchange and size-exclusion chromatography, A B-liter culture of induced E. coli provided 37 mg of purified PBP2a which was greater than 99% pure, finding affinities for [H-3]benzylpenicillin, imipenem, and L-695,256 (a beta-lactam with high affinity for PBP2a) were shown to be comparable to PEP2a found in membrane preparations of S. aureus strain 27R, A direct binding assay, using C-14-labeled L-695,256 was developed and used to show stoichiometric binding to the refolded, soluble PBP2a, In addition, electrospray mass spectrometry showed that 100% of the refolded PBP2a was covalently bound to the beta-lactam in a stoichiometric fashion. Finally, two mutations of the putative active-site serine showed the predicted loss of covalent binding of the beta-lactam to the PBP2a, demonstrating the high specificity of the soluble binding assay. (C) 1995 Academic Press, Inc.