IMMUNOREACTIVITY OF ADSORBED ANTIHUMAN CHORIONIC-GONADOTROPIN STUDIED WITH AN OPTICAL WAVE-GUIDE INTERFEROMETRIC SENSOR

被引:15
作者
HEIDEMAN, RG [1 ]
KOOYMAN, RPH [1 ]
GREVE, J [1 ]
机构
[1] TWENTE UNIV TECHNOL,DEPT APPL PHYS,APPL OPT GRP,MESA RES INST,POB 217,7500 AE ENSCHEDE,NETHERLANDS
关键词
IMMUNOSENSOR; IMMUNOREACTIVITY; ADSORBED ANTIBODIES; INTERFEROMETER; PLANAR WAVE-GUIDES; HYDROPHOBICITY; COVERAGE;
D O I
10.1016/0956-5663(94)80013-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A study on the immunoreactivity of adsorbed alphahCG molecules as a function of substrate hydrophobicity and protein coverage is presented. The experiments were performed with a planar waveguide interferometrical immunosensor. The substrate hydrophobicity and the antibody density were found to be of major importance for the immunoreactivity of the adsorbed antibodies. A combination of a hydrophobic (contact angle greater-than-or-equal-to 80-degrees) substrate and only a fraction (almost-equal-to 3) of a monolayer of antibodies gives the highest immunoreactivity. However, the immunoreaction affinity constant of the active antibodies is found to be essentially independent of the substrate hydrophobicity. The experimental results indicate that the orientation and not the conformation of the active antibodies on the substrate is dependent on the substrate hydrophobicity.
引用
收藏
页码:33 / 43
页数:11
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