IMMUNODOMINANT ANTIGENIC REGIONS IN A STRUCTURAL PROTEIN OF THE HEPATITIS-E VIRUS

被引:65
作者
KHUDYAKOV, YE
FAVOROV, MO
JUE, DL
HINE, TK
FIELDS, HA
机构
[1] CTR DIS CONTROL & PREVENT, NATL CTR INFECT DIS, HEPATITIS BRANCH, ATLANTA, GA 30333 USA
[2] CTR DIS CONTROL & PREVENT, NATL CTR INFECT DIS, BIOTECHNOL CORE FACIL BRANCH, ATLANTA, GA 30333 USA
[3] DI IVANOVSKII INST VIROL, MOSCOW 123098, RUSSIA
关键词
D O I
10.1006/viro.1994.1048
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using a set of 11 synthetic peptides containing regions of the polypeptide encoded by open reading frame 2 (ORF2) of the hepatitis E virus (HEV) genomic RNA, two immunodominant regions were found. One region is located at position 546-580 amino acids (aa). Another very strong immunodominant region was identified at position 394-470 aa. Five peptides spanning this region were found to have antigenic reactivity. One of these 5 peptides demonstrated specific reactivity with 81% of sera obtained from HEV-infected patients. To elucidate the antigenic structure of this region in fine detail, an additional set of 35 overlapped 20-mer peptides spanning the region 388-493 aa was synthesized. Two subregions with very strong antigenic reactivity, one located around position 420-440 aa and another around 450-460 aa, were identified. Thus, in addition to the strong epitope(s) in the C-terminal region of the ORF2 protein previously identified, at least two immunodominant regions were found at positions 394-470 and 546-580 aa. © 1994 Academic Press, Inc.
引用
收藏
页码:390 / 393
页数:4
相关论文
共 14 条
  • [1] SEQUENCE AND GENE STRUCTURE OF THE HEPATITIS-E VIRUS ISOLATED FROM MYANMAR
    AYE, TT
    UCHIDA, T
    MA, XZ
    IIDA, F
    SHIKATA, T
    ICHIKAWA, M
    RIKIHISA, T
    WIN, KM
    [J]. VIRUS GENES, 1993, 7 (01) : 95 - 110
  • [2] BRADLEY DW, 1990, PROG MED VIROL, V37, P101
  • [3] SOLID-PHASE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HEPATITIS-E VIRUS IGG AND IGM ANTIBODIES UTILIZING RECOMBINANT ANTIGENS AND SYNTHETIC PEPTIDES
    DAWSON, GJ
    CHAU, KH
    CABAL, CM
    YARBOUGH, PO
    REYES, GR
    MUSHAHWAR, IK
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1992, 38 (01) : 175 - 186
  • [4] SEROLOGIC IDENTIFICATION OF HEPATITIS-E VIRUS-INFECTIONS IN EPIDEMIC AND ENDEMIC SETTINGS
    FAVOROV, MO
    FIELDS, HA
    PURDY, MA
    YASHINA, TL
    ALEKSANDROV, AG
    ALTER, MJ
    YARASHEVA, DM
    BRADLEY, DW
    MARGOLIS, HS
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1992, 36 (04) : 246 - 250
  • [5] ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DIAGNOSIS OF ACUTE SPORADIC HEPATITIS-E IN EGYPTIAN CHILDREN
    GOLDSMITH, R
    YARBOUGH, PO
    REYES, GR
    FRY, KE
    GABOR, KA
    KAMEL, M
    ZAKARIA, S
    AMER, S
    GAFFAR, Y
    [J]. LANCET, 1992, 339 (8789) : 328 - 331
  • [6] CLONING AND EXPRESSION OF CDNAS FROM ENTERICALLY-TRANSMITTED NON-A, NON-B HEPATITIS-VIRUS
    ICHIKAWA, M
    ARAKI, M
    RIKIHISA, T
    UCHIDA, T
    SHIKATA, T
    MIZUNO, K
    [J]. MICROBIOLOGY AND IMMUNOLOGY, 1991, 35 (07) : 535 - 543
  • [7] HUMAN LINEAR B-CELL EPITOPES ENCODED BY THE HEPATITIS-E VIRUS INCLUDE DETERMINANTS IN THE RNA-DEPENDENT RNA-POLYMERASE
    KAUR, M
    HYAMS, KC
    PURDY, MA
    KRAWCZYNSKI, K
    CHING, WM
    FRY, KE
    REYES, GR
    BRADLEY, DW
    CARL, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) : 3855 - 3858
  • [8] EPITOPE MAPPING IN PROTEINS OF HEPATITIS-E VIRUS
    KHUDYAKOV, YE
    KHUDYAKOVA, NS
    FIELDS, HA
    JUE, D
    STARLING, C
    FAVOROV, MO
    KRAWCZYNSKI, K
    POLISH, L
    MAST, E
    MARGOLIS, H
    [J]. VIROLOGY, 1993, 194 (01) : 89 - 96
  • [9] REYES GR, 1991, GASTROENTEROL JPN, V26, P142
  • [10] REYES GR, 1991, INT CONGR SER, V895, P237