CLONING AND EXPRESSION OF LARGE ISOFORM OF GLUTAMIC-ACID DECARBOXYLASE FROM HUMAN PANCREATIC-ISLET

被引：14

作者：

KAWASAKI, E

MORIUCHI, R

WATANABE, M

SAITOH, K

BRUNICARDI, FC

WATT, PC

YAMAGUCHI, T

MULLEN, Y

AKAZAWA, S

MIYAMOTO, T

NAGATAKI, S

机构：

[1] NAGASAKI UNIV, SCH MED, DEPT BACTERIOL, NAGASAKI 852, JAPAN

[2] MITSUBISHI PETR CO LTD, TSUKUBA RES CTR, IBARAKI 30003, JAPAN

[3] UNIV CALIF LOS ANGELES, DEPT SURG, LOS ANGELES, CA 90024 USA

[4] UNIV CALIF LOS ANGELES, DIABET RES CTR, LOS ANGELES, CA 90024 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 192卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1565

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutamic acid decarboxylase (GAD) catalyzes formation of γ-aminobutyric acid from glutamic acid and is a major autoantigen in insulin-dependent diabetes mellitus. Its two isoforms, GAD65 and GAD67, are encoded by two separate genes. We prepared human islet cDNA library and screened it with cDNA probes of rat brain GAD67. We cloned the cDNA for GAD67, the large isoform of glutamic acid decarboxylase, and determined its nucleotide sequence. Sequencing of the resulting clone identified a 1,785 residue open- reading frame encoded a 594 amino acid polypeptide that showed a 99.4% similarity with GAD67 from human brain. The bacterially expressed human islet GAD67 protein was enzymatically active and immunoreactive. The isolation of cDNA for this additional islet GAD isoforms will be important in studying the etiology and pathogenesis of IDDM. © 1993 Academic Press, Inc.

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页码：1353 / 1359

页数：7

相关论文

共 23 条

[1] 64000 MR AUTOANTIBODIES AS PREDICTORS OF INSULIN-DEPENDENT DIABETES [J].

ATKINSON, MA ;

MACLAREN, NK ;

SCHARP, DW ;

LACY, PE ;

RILEY, WJ .

LANCET, 1990, 335 (8702) :1357-1360

[2] RESPONSE OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS TO GLUTAMATE-DECARBOXYLASE IN INSULIN-DEPENDENT DIABETES [J].

ATKINSON, MA ;

KAUFMAN, DL ;

CAMPBELL, L ;

GIBBS, KA ;

SHAH, SC ;

BU, DF ;

ERLANDER, MG ;

TOBIN, AJ ;

MACLAREN, NK .

LANCET, 1992, 339 (8791) :458-459

[3] AUTOANTIBODIES IN NONOBESE DIABETIC MICE IMMUNOPRECIPITATE 64,000-MR ISLET ANTIGEN [J].

ATKINSON, MA ;

MACLAREN, NK .

DIABETES, 1988, 37 (11) :1587-1590

[4] AUTOANTIBODIES TO A 64-KILODALTON ISLET CELL PROTEIN PRECEDE THE ONSET OF SPONTANEOUS DIABETES IN THE BB RAT [J].

BAEKKESKOV, S ;

DYRBERG, T ;

LERNMARK, A .

SCIENCE, 1984, 224 (4655) :1348-1350

[5] ANTIBODIES TO A 64,000 MR HUMAN ISLET CELL ANTIGEN PRECEDE THE CLINICAL ONSET OF INSULIN-DEPENDENT DIABETES [J].

BAEKKESKOV, S ;

LANDIN, M ;

KRISTENSEN, JK ;

SRIKANTA, S ;

BRUINING, GJ ;

MANDRUPPOULSEN, T ;

DEBEAUFORT, C ;

SOELDNER, JS ;

EISENBARTH, G ;

LINDGREN, F ;

SUNDQUIST, G ;

LERNMARK, A .

JOURNAL OF CLINICAL INVESTIGATION, 1987, 79 (03) :926-934

[6] IDENTIFICATION OF THE 64K AUTOANTIGEN IN INSULIN-DEPENDENT DIABETES AS THE GABA-SYNTHESIZING ENZYME GLUTAMIC-ACID DECARBOXYLASE [J].

BAEKKESKOV, S ;

AANSTOOT, HJ ;

CHRISTGAU, S ;

REETZ, A ;

SOLIMENA, M ;

CASCALHO, M ;

FOLLI, F ;

RICHTEROLESEN, H ;

CAMILLI, PD .

NATURE, 1990, 347 (6289) :151-156

[7]

BOTTAZZO GF, 1987, DIABETES ANN, V3, P15

[8] 2 HUMAN GLUTAMATE DECARBOXYLASES, 65-KDA GAD AND 67-KDA GAD, ARE EACH ENCODED BY A SINGLE GENE [J].

BU, DF ;

ERLANDER, MG ;

HITZ, BC ;

TILLAKARATNE, NJK ;

KAUFMAN, DL ;

WAGNERMCPHERSON, CB ;

EVANS, GA ;

TOBIN, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2115-2119

[9] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[10] CLONING AND PARTIAL NUCLEOTIDE-SEQUENCE OF HUMAN GLUTAMIC-ACID DECARBOXYLASE CDNA FROM BRAIN AND PANCREATIC-ISLETS [J].

CRAM, DS ;

BARNETT, LD ;

JOSEPH, JL ;

HARRISON, LC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (03) :1239-1244