ISOLATION AND PARTIAL-PURIFICATION OF CARTHAMINE - AN INSTRUMENTATION MANUAL

Crude carthamine was obtainable through alkaline extraction, acidification, and cellulose adsorption. It was purified by column chromatography on Avicel cellulose and Toyo Pearl HW-40f. Using these techniques, the dye was purified up to about 3.5-fold at a final yield of 28.4% (mg carthamine.g dry flower-1: 5.22-->1.48). On the basis of the experimental model data, an instruction manual for isolation and purification of carthamine is presented in order to standarize the bio-dye preparation at an economically pertinent cost.