DETERMINATION OF N-ACETYLGLUCOSAMINYLTRANSFERASE-III, N-ACETYLGLUCOSAMINYLTRANSFERASE-IV AND N-ACETYLGLUCOSAMINYLTRANSFERASE-V IN NORMAL AND HEPATOMA TISSUES OF RATS

被引：109

作者：

NISHIKAWA, A ^{[1
]}

GU, J ^{[1
]}

FUJII, S ^{[1
]}

TANIGUCHI, N ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT BIOCHEM,4-3-57 NAKANOSHIMA,KITA KU,TOYONAKA,OSAKA 560,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1035卷 / 03期

关键词：

(Rat); 2-Aminopyridine; HPLC; N-Acetylglucosaminyltransferase;

D O I：

10.1016/0304-4165(90)90094-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Acetylglucosaminyltransferase III, IV and V activities were assayed in various rat tissues and hepatomas using the same fluorescence-labeled sugar chain, GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc-2-aminopyridine as a substrate. The N-acetylglucosaminyltransferase II activity toward the substrate is the highest in most rat tissues including primary rat hepatoma. A relatively higher activity for GnT-V is found in small intestine, serum and hepatoma as compared to that of GnT-IV. Some kinetic properties of these enzymes in crude extracts were also determined. © 1990.

引用

页码：313 / 318

页数：6

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