A C-TERMINAL PROLINE IS REQUIRED FOR BIOLUMINESCENCE OF THE CA-2+-BINDING PHOTOPROTEIN, AEQUORIN

被引：64

作者：

NOMURA, M

INOUYE, S

OHMIYA, Y

TSUJI, FI

机构：

[1] OSAKA BIOSCI INST,6-2-4 FURUEDAI,SUITA,OSAKA 565,JAPAN

[2] CHISSO CORP,YOKOHAMA RES CTR,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

[3] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV MARINE BIOL RES 0202,LA JOLLA,CA 92093

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

AEQUORIN; CA2+-BINDING PROTEIN; BIOLUMINESCENCE; CARBOXYL-TERMINUS; PROLINE; DELETION MUTANT;

D O I：

10.1016/0014-5793(91)81385-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The requirement for a proline residue at the C-terminus of the Ca2+-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.

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页码：63 / 66

页数：4

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