A C-TERMINAL PROLINE IS REQUIRED FOR BIOLUMINESCENCE OF THE CA-2+-BINDING PHOTOPROTEIN, AEQUORIN

被引:64
作者
NOMURA, M
INOUYE, S
OHMIYA, Y
TSUJI, FI
机构
[1] OSAKA BIOSCI INST,6-2-4 FURUEDAI,SUITA,OSAKA 565,JAPAN
[2] CHISSO CORP,YOKOHAMA RES CTR,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
[3] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV MARINE BIOL RES 0202,LA JOLLA,CA 92093
关键词
AEQUORIN; CA2+-BINDING PROTEIN; BIOLUMINESCENCE; CARBOXYL-TERMINUS; PROLINE; DELETION MUTANT;
D O I
10.1016/0014-5793(91)81385-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The requirement for a proline residue at the C-terminus of the Ca2+-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.
引用
收藏
页码:63 / 66
页数:4
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