REACTIVITY OF [FE] AND [NI-FE-SE] HYDROGENASES WITH THEIR OXIDOREDUCTION PARTNER - THE TETRAHEME CYTOCHROME-C3

In order to understand the electron transfer mechanisms for the [Fe] and [NiFe] hydrogenases, a kinetic study of cytochrome c3 reduction has been undertaken. Cyclic voltammetry and controlled-potential amperometry techniques have been used to investigate the intermolecular electron-transfer reaction between cytochrome c3 and [Fe] hydrogenase from Desulfovibrio vulgaris Hildenborough. Electron-transfer cross-reactions between [Fe] or [NiFeSe] hydrogenase and cytochrome c3 from Desulfovibrio vulgaris Hildenborough or Desulfovibrio desulfuricans Norway have been studied. Some structural implications are considered from these experimental data. © 1992.