INACTIVATION AND REACTIVATION OF MANGANESE CATALASE - OXIDATION-STATE ASSIGNMENTS USING X-RAY ABSORPTION-SPECTROSCOPY

被引:61
作者
WALDO, GS [1 ]
FRONKO, RM [1 ]
PENNERHAHN, JE [1 ]
机构
[1] UNIV MICHIGAN, DEPT CHEM, 930 N UNIV, ANN ARBOR, MI 48109 USA
关键词
D O I
10.1021/bi00107a017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The oxidation states of the Mn atoms in three derivatives of Mn catalase have been characterized using a combination of X-ray absorption near-edge structure (XANES) and EPR spectroscopies. The as-isolated enzyme has an average oxidation state of Mn(III) and contains a Mn(III) form, together with a reduced Mn(II) form and a variable amount (10-25%) of a Mn(III)/Mn(IV) mixed-valence derivative. Treatment with NH2OH rapidly reduces the majority of the enzyme to a Mn(II) derivative with no loss of activity. Inactivation by treatment with NH2OH + H2O2 converts all of the enzyme to a mixed-valence Mn(III)/Mn(IV) form. The inactive, mixed-valence derivative can be completely reactivated by long-term (> 1 h) anaerobic incubation with NH2OH, giving a reduced Mn(II)/Mn(II) derivative. These data suggest a catalytic model in which the enzyme cycles between a reduced Mn(II)/Mn(II) state and an oxidized Mn(III)/Mn(III) state.
引用
收藏
页码:10486 / 10490
页数:5
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