2-DIMENSIONAL CRYSTALLIZATION OF THE LIGHT-HARVESTING COMPLEX FROM RHODOSPIRILLUM-RUBRUM

被引:26
作者
GHOSH, R
HOENGER, A
HARDMEYER, A
MIHAILESCU, D
BACHOFEN, R
ENGEL, A
ROSENBUSCH, JP
机构
[1] UNIV BASEL, BIOCTR, ME MULLER INST HIGH RESOLUT ELECTRON MICROSCOPY, CH-4056 BASEL, SWITZERLAND
[2] INST PLANT BIOL, CH-8008 ZURICH, SWITZERLAND
关键词
2D-CRYSTALS; MEMBRANES; LIGHT-HARVESTING COMPLEXES; RHODOSPIRILLUM-RUBRUM;
D O I
10.1006/jmbi.1993.1298
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phospholipid per mol αβ dimer, the arrays formed by a self assembly process are primary due to protein-protein interactions. The hexagonal lattices were analyzed by negative stain electron microscopy and digital image processing. They exhibited a unit cell size of 12.3 nm, in close agreement with the particle diameter of the active photo-unit in native chromatophore membranes. The unit cell contains a central 5 nm stain-filled depression, embraced by a ring with an outer diameter of 10 nm. © 1993 Academic Press, Inc.
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页码:501 / 504
页数:4
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