SIMULTANEOUS GC DERIVATIZATION AND QUANTIFICATION OF ACIDS AND SUGARS

N-methoxy-N,O-bistrimethylsilyl carbamate (BSMOC) has been introduced as a 'one step' derivatizing agent to determine nonvolatile acids and sugars in a single injection by gas-liquid chromatography. A systematic study has been carried out to optimize the derivatization conditions. Reproducibility data showed that > 0.04 mug of acids in the presence of < 6 mug of sugars can be measured with a relative standard error (R.S.E.) of 6.5 % or less. After a simple preparation of fresh apple sample, sugars were converted to their methoxime-trimethylsilyl and the acids to their trimethylsilyl derivatives, with BSMOC, simultaneously.