3-DIMENSIONAL MODELS OF HUMAN AND OTHER MAMMALIAN MICROSOMAL P450S CONSTRUCTED FROM AN ALIGNMENT WITH P450102 (P450(BM3))

被引：86

作者：

LEWIS, DFV

机构：

[1] Molecular Toxicology Group, School of Biological Sciences, University of Surrey, Guildford

来源：

XENOBIOTICA | 1995年 / 25卷 / 04期

关键词：

D O I：

10.3109/00498259509061857

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. A novel modelling alignment for P450s, utilizing NADPH-P450 reductase for electron transfer, is proposed on the basis of analysis of their amino acid sequences. 2. Information used to facilitate the alignment process includes: the recent X-ray crystal structure of P450102 (P450(bm3)), site-directed mutagenesis experiments, chemical modification of specific residues, and antibody recognition studies. 3. The alignment has been used to construct a number of microsomal P450s of relevance to xenobiotic and endogenous metabolism.

引用

页码：333 / 366

页数：34

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