IDENTIFICATION OF A CATALYTICALLY ESSENTIAL NUCLEOPHILIC RESIDUE IN SHEEP LIVER CYTOPLASMIC ALDEHYDE DEHYDROGENASE

被引：37

作者：

KITSON, TM ^{[1
]}

HILL, JP ^{[1
]}

MIDWINTER, GG ^{[1
]}

机构：

[1] UNIV HULL,DEPT BIOCHEM,HULL HU6 7RX,N HUMBERSIDE,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[C-14]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.

引用

页码：207 / 210

页数：4

共 26 条

[11] CLONING AND COMPLETE NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA-ENCODING A CATALYTICALLY FUNCTIONAL TUMOR-ASSOCIATED ALDEHYDE DEHYDROGENASE
JONES, DE
BRENNAN, MD
HEMPEL, J
LINDAHL, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) : 1782 - 1786
[12] EFFECT OF DISULFIRAM ON THE PRE-STEADY-STATE BURST IN THE REACTIONS OF SHEEP LIVER CYTOPLASMIC ALDEHYDE DEHYDROGENASE
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1987, 248 (03) : 989 - 991
[13] KINETICS OF P-NITROPHENYL PIVALATE HYDROLYSIS CATALYZED BY CYTOPLASMIC ALDEHYDE DEHYDROGENASE
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1989, 257 (02) : 573 - 578
[14] STUDIES ON INTERACTION BETWEEN DISULFIRAM AND SHEEP LIVER CYTOPLASMIC ALDEHYDE DEHYDROGENASE
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1978, 175 (01) : 83 - 90
[15] THE ACTION OF CYTOPLASMIC ALDEHYDE DEHYDROGENASE ON METHYL PARA-NITROPHENYL CARBONATE AND PARA-NITROPHENYL DIMETHYLCARBAMATE
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1989, 257 (02) : 579 - 584
[16] THE BINDING OF NADH TO CYTOPLASMIC ALDEHYDE DEHYDROGENASE AFTER MODIFICATION WITH PARA-NITROPHENYL DIMETHYLCARBAMATE
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1989, 257 (02) : 585 - 590
[17] KOK M, 1989, J BIOL CHEM, V264, P5442
[18] EVIDENCE FOR REACTIVITY OF SERINE-74 WITH TRANS-4-(N,N-DIMETHYLAMINO)CINNAMALDEHYDE DURING OXIDATION BY THE CYTOPLASMIC ALDEHYDE DEHYDROGENASE FROM SHEEP LIVER
LOOMES, KM
MIDWINTER, GG
BLACKWELL, LF
BUCKLEY, PD
[J]. BIOCHEMISTRY, 1990, 29 (08) : 2070 - 2075
[19] ALDEHYDE DEHYDROGENASE CATALYZES ACETALDEHYDE FORMATION FROM 4-NITROPHENYL ACETATE AND NADH
LOOMES, KM
KITSON, TM
[J]. BIOCHEMICAL JOURNAL, 1986, 238 (02) : 617 - 619
[20] EVIDENCE THAT THE CYTOPLASMIC ALDEHYDE DEHYDROGENASE-CATALYZED OXIDATION OF ALDEHYDES INVOLVES A DIFFERENT ACTIVE-SITE GROUP FROM THAT WHICH CATALYZES THE HYDROLYSIS OF 4-NITROPHENYL ACETATE
MOTION, RL
BUCKLEY, PD
BENNETT, AF
BLACKWELL, LF
[J]. BIOCHEMICAL JOURNAL, 1988, 254 (03) : 903 - 906

← 1 2 3 →