DETERMINATION OF L-MALATE IN WINE BY FLOW-INJECTION WITH CO-IMMOBILIZED MALATE-DEHYDROGENASE OXALOACETATE DECARBOXYLASE

被引：11

作者：

KIBA, N

OGUCHI, M

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

TALANTA | 1993年 / 40卷 / 08期

关键词：

D O I：

10.1016/0039-9140(93)80182-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for determination of L-Malate in wine is described. Malate dehydrogenase and oxaloacetate decarboxylase were immobilized on poly (vinyl alcohol) beads and incorporated in a flow-injection system with fluorescence detection. Sample solution (50 mul) was injected into the carrier stream [4mM NAD+ in glycine buffer (pH 10.0)]. The fluorescence based on the NADH formed could be directly related to the amount of malate. The calibration graph was linear over the range 0.4-300 muM. The detection limit was 0.2 muM. Sampling throughout was 25 samples/hr.

引用

页码：1163 / 1165

页数：3

共 12 条

[1] FLOW-INJECTION DETERMINATION OF MALATE WITH IMMOBILIZED MALATE-DEHYDROGENASE [J].