CLONING OF FRK, A NOVEL HUMAN INTRACELLULAR SRC-LIKE TYROSINE KINASE-ENCODING GENE

被引：53

作者：

LEE, J

WANG, ZY

LUOH, SM

WOOD, WI

SCADDEN, DT

机构：

[1] NEW ENGLAND DEACONESS HOSP,HARVARD MED SCH,DIV HEMATOL ONCOL,BOSTON,MA 02215

[2] GENENTECH INC,S SAN FRANCISCO,CA 94080

来源：

GENE | 1994年 / 138卷 / 1-2期

关键词：

SIGNAL TRANSDUCTION; PROLIFERATION CONTROLS; DIFFERENTIATION CONTROLS; CELL REGULATION; ONCOGENES;

D O I：

10.1016/0378-1119(94)90817-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related kinase or FRK, is a novel member of the intracellular TYK gene family.

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页码：247 / 251

页数：5

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