CHARACTERIZATION OF A 2ND PROMOTER FOR THE MOUSE-LIVER BONE KIDNEY-TYPE ALKALINE-PHOSPHATASE GENE - CELL AND TISSUE SPECIFIC EXPRESSION

被引：30

作者：

STUDER, M ^{[1
]}

TERAO, M ^{[1
]}

GIANNI, M ^{[1
]}

GARATTINI, E ^{[1
]}

机构：

[1] MARIO NEGRI INST PHARMACOL RES,CTR DANIELA & CATULLO BORGOMAINERIO,MOLEC BIOL UNIT,I-20157 MILAN,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91722-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines of different origin and in various tissues by polymerase chain reaction and RNase mapping analysis. The first promoter is active in embryo derived cells, whereas the second promoter is silent in basal conditions but it is activated by dibutyryl cAMP in fibroblastic cells. In the whole animal, the transcript driven by the first promoter is found in most tissues albeit at different levels, while the one driven by the second promoter is specifically expressed at high levels only in the heart. © 1991.

引用

页码：1352 / 1360

页数：9

共 27 条

[1] SIMIAN VIRUS-40 MAJOR LATE PROMOTER - A NOVEL TRIPARTITE STRUCTURE THAT INCLUDES INTRAGENIC SEQUENCES [J].