FASCIOLA-HEPATICA - A SECRETED CATHEPSIN L-LIKE PROTEINASE CLEAVES HOST IMMUNOGLOBULIN

被引：94

作者：

SMITH, AM ^{[1
]}

DOWD, AJ ^{[1
]}

HEFFERNAN, M ^{[1
]}

ROBERTSON, CD ^{[1
]}

DALTON, JP ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT ZOOL,BIOCHEM PARASITOL LAB,GLASGOW G12 8QQ,SCOTLAND

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 08期

基金：

英国惠康基金;

关键词：

FASCIOLA-HEPATICA; IN-VITRO RELEASED PRODUCTS; CYSTEINE PROTEINASES; CATHEPSIN-L; PAPAIN; TREMATODE;

D O I：

10.1016/0020-7519(93)90117-H

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Adult Fasciola hepatica secrete a cysteine proteinase capable of cleaving host IgG close to the papain cleaving site. The proteinase was separated by size permeation chromatography. Gelatin-substrate polyacrylamide gel electrophoresis analysis revealed that the proteinase migrates as 6 proteolytic bands in the apparent molecular size range 60-90 kDa. Based on pH profiles of activity, inhibition studies using diethylpyrocarbonate and the diazomethylketone Z-phe-ala-CHN2, and characterising the substrate specificity of the enzymes using fluorogenic peptide substrates we have shown that the 60-90-kDa proteinases are cathepsin L-like proteinases.

引用

页码：977 / 983

页数：7