SIMPLE KINETIC-MODELS FOR PROTEIN EXCHANGE-REACTIONS ON SOLID-SURFACES

Kinetic models for the exchange reactions between protein molecules adsorbed on a solid surface and in solution are developed. The expected protein adsorption kinetics is calculated. Dynamic phenomena taking place on the surface are considered in the sense that molecules may be transformed into a nonexchangeable form. Some microscopic details related to spontaneous desorption and exchange reactions are discussed. Some general conclusions are made about different types of surfaces. It is thus concluded that a surface may cause unwanted biological effects due to both a too strong binding of protein molecules and a too weak binding. The results obtained from the model calculations are in qualitative agreement with experimental results. They particularly emphasize the importance of slow dynamic phenomena taking place after the initial adsorption of protein molecules. © 1990.