ROLE OF SOLVENTS IN THE RESOLUTION OF SOME BETA-ADRENERGIC BLOCKERS ON CELLULOSE 3,5-DIMETHYLPHENYL CARBAMATE CHIRAL STATIONARY PHASE

The stereoselective resolution of racemic mixture of seven beta-adrenergic blockers were achieved on cellulose 3,5-diemethylphenyl carbamate chiral stationary phase (Chiralcel OD). The role of the mobile phase solvents in the resolution of these beta-blockers, namely, tertalolol, oxprenolol, p-oxprenolol, alprenolol, acebutolol, bisoprolol and tolamolol were presented. It was found that it is not essential to add diethylamine as a suppressor in the mobile phase to effect the separation of the racemate to their corresponding enantiomers. However, addition of diethylamine improved the symmetry and sharpness of the peaks. The chiral recognition mechanism(s) of this phase is discussed.