MOLECULAR CHARACTERIZATION OF A RECURRING COMPLEX CHROMOSOMAL TRANSLOCATION IN 2 HUMAN EXTRAGONADAL GERM-CELL TUMORS

被引：22

作者：

SINKE, RJ

WEGHUIS, DO

SUIJKERBUIJK, RF

TANIGAMI, A

NAKAMURA, Y

LARSSON, C

WEBER, G

DEJONG, B

OOSTERHUIS, JW

MOLENAAR, WM

VANKESSEL, AG

机构：

[1] CANC INST, DIV BIOCHEM, TOKYO, JAPAN

[2] KAROLINSKA INST, DEPT CLIN GENET, STOCKHOLM, SWEDEN

[3] UNIV GRONINGEN, DEPT HUMAN GENET & PATHOL, GRONINGEN, NETHERLANDS

[4] DR DANIEL DEN HOED CANC CTR, ROTTERDAM, NETHERLANDS

来源：

CANCER GENETICS AND CYTOGENETICS | 1994年 / 73卷 / 01期

关键词：

D O I：

10.1016/0165-4608(94)90176-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6p23, and 11q13 in two independent bur similar extragonadal human germ cell rumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).

引用

页码：11 / 16

页数：6

共 28 条

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