CLONING, SEQUENCE AND STRUCTURAL FEATURES OF A LIPASE FROM THE ANTARCTIC FACULTATIVE PSYCHROPHILE PSYCHOROBACTER-IMMOBILIS B10

被引：59

作者：

ARPIGNY, JL

FELLER, G

GERDAY, C

机构：

[1] Laboratoire de Biochimie, Institut de Chimie B6, Université de Liège-Sart Tilman, Liège

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1171卷 / 03期

关键词：

LIPASE; FACULTATIVE PSYCHROPHILE; DNA SEQUENCE; ANTARCTIC; COLD ADAPTATION;

D O I：

10.1016/0167-4781(93)90078-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35 288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.

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页码：331 / 333

页数：3

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