ACTIVITY, ISOENZYMES AND PURITY OF MUSHROOM TYROSINASE IN COMMERCIAL PREPARATIONS

被引：32

作者：

KUMAR, M ^{[1
]}

FLURKEY, WH ^{[1
]}

机构：

[1] INDIANA STATE UNIV, DEPT CHEM, TERRE HAUTE, IN 47809 USA

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 12期

关键词：

AGARCIUS-BISPORUS; MUSHROOM; TYROSINASE; ISOENZYMES; PURITY; COMMERCIAL PREPARATIONS;

D O I：

10.1016/0031-9422(91)83430-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several lots of commercial tyrosinase preparations were examined with regard to their enzyme activity, isoenzyme composition and purity. Enzyme activity toward catechol, L-dopa and tyrosine showed significant variations from lot to lot and activation by SDS. Distribution of isoenzyme forms also varied from lot to lot. Comparisons of electrophoretic and isoelectric focusing protein profiles showed considerable differences and distributions of the proteins in each sample. Tyrosinase appeared to be a minor component in each preparation when compared to a partially purified enzyme. Investigators using commercial tyrosinase should exercise caution in interpreting data due to the presence of different isoenzyme forms, their distribution in various lots, and the presence of numerous other proteins.

引用

页码：3899 / 3902

页数：4

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