INHIBITION OF PHOTOSYNTHETIC ELECTRON-TRANSPORT IN SPINACH-CHLOROPLASTS BY LOW OSMOLARITY

被引：37

作者：

LOCKAU, W

机构：

[1] Institut Für Botanik, Fachbereich Biologie Und Vorklinische Medizin Der Universität Regensburg, Regensburg, D-8400

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb12902.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reversible inhibition, by low osmolarity, of the rate of electron transport through photosystem 1 has been investigated in spinach chloroplasts. By use of different electron donor systems to photosystem 1, inhibitors of plastocyanin, and by measurement of the extent of photooxidation of the photosystem 1 reaction center P700, the inhibition site has been localized on the electron donor side of this photosystem. From comparison of the influence of impermeant and permeant salts on the electron transport rate, and from the effect of ionic strength on the oxidation of externally added plastocyanin by subchloroplast preparations, it is concluded that low ionic strength within the thylakoids inhibits the photooxidation of endogenous plastocyanin by P700. The results are taken as evidence that plastocyanin is oxidized by P700 at the internal (lumen) side of the osmotic barrier in the thylakoid membrane. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：365 / 373

页数：9

共 38 条

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