THE PHOSPHOENOLPYRUVATE CARBOXYLASE GENE FAMILY OF SORGHUM - PROMOTER STRUCTURES, AMINO-ACID-SEQUENCES AND EXPRESSION OF GENES

被引：67

作者：

CRETIN, C ^{[1
]}

SANTI, S ^{[1
]}

KERYER, E ^{[1
]}

LEPINIEC, L ^{[1
]}

TAGU, D ^{[1
]}

VIDAL, J ^{[1
]}

GADAL, P ^{[1
]}

机构：

[1] UNIV UDINE,IST PROD VEGETALE,I-33100 UDINE,ITALY

来源：

GENE | 1991年 / 99卷 / 01期

关键词：

C4; PHOTOSYNTHESIS; RECOMBINANT DNA; GENOMIC LIBRARY; PHAGE-LAMBDA VECTOR; PROMOTER SEQUENCE; S1; MAPPING;

D O I：

10.1016/0378-1119(91)90037-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two different members of the phosphoenolpyruvate carboxylase(PEPC)-encoding multigene family (clones lambda-CP21 and lambda-CP46) have been isolated from a Sorghum vulgare lambda-EMBL4 genomic library. The use of the 3'-noncoding regions to probe Northern blots of RNA from roots, etiolated leaves and green leaves indicated that lambda-CP21 and lambda-CP46 encode the C3- and C4-type leaf PEPC isoforms, respectively. The lambda-CP21 clone is expressed in the three tissues and is not light-regulated, whereas lambda-CP46 is only expressed in greening leaves. The nucleotide sequence of the 5'-flanking DNA (520 bp) has been determined for both genes. For lambda-CP46, several direct repeats were located in this region with similarities to sequences found in other light-regulated genes, but not in lambda-CP21. The deduced amino acid sequences of the two S. vulgare PEPC proteins are 75% identical.

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页码：87 / 94

页数：8

相关论文

共 51 条

[1] UNMETHYLATED CPG ISLANDS ASSOCIATED WITH GENES IN HIGHER-PLANT DNA [J].

ANTEQUERA, F ;

BIRD, AP .

EMBO JOURNAL, 1988, 7 (08) :2295-2299

[2] CPG ISLANDS AS GENE MARKERS IN THE VERTEBRATE NUCLEUS [J].

BIRD, AP .

TRENDS IN GENETICS, 1987, 3 (12) :342-347

[3] STRUCTURAL-ANALYSIS OF NUCLEAR GENES-CODING FOR THE PRECURSOR TO THE SMALL SUBUNIT OF WHEAT RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE [J].

BROGLIE, R ;

CORUZZI, G ;

LAMPPA, G ;

KEITH, B ;

CHUA, NH .

BIO-TECHNOLOGY, 1983, 1 (01) :55-61

[4] DNA METHYLATION AND GENE ACTIVITY [J].

CEDAR, H .

CELL, 1988, 53 (01) :3-4

[5] IDENTIFICATION OF A CDNA CLONE FOR SORGHUM LEAF MALATE-DEHYDROGENASE (NADP) - LIGHT-DEPENDENT MESSENGER-RNA ACCUMULATION [J].

CRETIN, C ;

LUCHETTA, P ;

JOLY, C ;

MIGINIACMASLOW, M ;

DECOTTIGNIES, P ;

JACQUOT, JP ;

VIDAL, J ;

GADAL, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 174 (03) :497-501

[6] COMPLETE CDNA SEQUENCE OF SORGHUM PHOSPHOENOLPYRUVATE CARBOXYLASE INVOLVED IN C4 PHOTOSYNTHESIS [J].

CRETIN, C ;

KERYER, E ;

TAGU, D ;

LEPINIEC, L ;

VIDAL, J ;

GADAL, P .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :658-658

[7] SALT STRESS LEADS TO DIFFERENTIAL EXPRESSION OF 2 ISOGENES OF PHOSPHOENOLPYRUVATE CARBOXYLASE DURING CRASSULACEAN ACID METABOLISM INDUCTION IN THE COMMON ICE PLANT [J].

CUSHMAN, JC ;

MEYER, G ;

MICHALOWSKI, CB ;

SCHMITT, JM ;

BOHNERT, HJ .

PLANT CELL, 1989, 1 (07) :715-725

[8] ORGAN-SPECIFIC AND LIGHT-INDUCED EXPRESSION OF PLANT GENES [J].

FLUHR, R ;

KUHLEMEIER, C ;

NAGY, F ;

CHUA, NH .

SCIENCE, 1986, 232 (4754) :1106-1112

[9] THE PRIMARY STRUCTURE OF PHOSPHOENOLPYRUVATE CARBOXYLASE OF ESCHERICHIA-COLI - NUCLEOTIDE-SEQUENCE OF THE PPC GENE AND DEDUCED AMINO-ACID-SEQUENCE [J].

FUJITA, N ;

MIWA, T ;

ISHIJIMA, S ;

IZUI, K ;

KATSUKI, H .

JOURNAL OF BIOCHEMISTRY, 1984, 95 (04) :909-916

[10] AN EVOLUTIONARILY CONSERVED PROTEIN-BINDING SEQUENCE UPSTREAM OF A PLANT LIGHT-REGULATED GENE [J].

GIULIANO, G ;

PICHERSKY, E ;

MALIK, VS ;

TIMKO, MP ;

SCOLNIK, PA ;

CASHMORE, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7089-7093

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