THE PHOSPHOENOLPYRUVATE CARBOXYLASE GENE FAMILY OF SORGHUM - PROMOTER STRUCTURES, AMINO-ACID-SEQUENCES AND EXPRESSION OF GENES

被引：67

作者：

CRETIN, C ^{[1
]}

SANTI, S ^{[1
]}

KERYER, E ^{[1
]}

LEPINIEC, L ^{[1
]}

TAGU, D ^{[1
]}

VIDAL, J ^{[1
]}

GADAL, P ^{[1
]}

机构：

[1] UNIV UDINE,IST PROD VEGETALE,I-33100 UDINE,ITALY

来源：

GENE | 1991年 / 99卷 / 01期

关键词：

C4; PHOTOSYNTHESIS; RECOMBINANT DNA; GENOMIC LIBRARY; PHAGE-LAMBDA VECTOR; PROMOTER SEQUENCE; S1; MAPPING;

D O I：

10.1016/0378-1119(91)90037-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two different members of the phosphoenolpyruvate carboxylase(PEPC)-encoding multigene family (clones lambda-CP21 and lambda-CP46) have been isolated from a Sorghum vulgare lambda-EMBL4 genomic library. The use of the 3'-noncoding regions to probe Northern blots of RNA from roots, etiolated leaves and green leaves indicated that lambda-CP21 and lambda-CP46 encode the C3- and C4-type leaf PEPC isoforms, respectively. The lambda-CP21 clone is expressed in the three tissues and is not light-regulated, whereas lambda-CP46 is only expressed in greening leaves. The nucleotide sequence of the 5'-flanking DNA (520 bp) has been determined for both genes. For lambda-CP46, several direct repeats were located in this region with similarities to sequences found in other light-regulated genes, but not in lambda-CP21. The deduced amino acid sequences of the two S. vulgare PEPC proteins are 75% identical.

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页码：87 / 94

页数：8

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