REGULATION OF THE SYNTHESIS OF UNSATURATED FATTY-ACIDS BY GROWTH TEMPERATURE IN BACILLUS-SUBTILIS

被引:47
作者
GRAU, R [1 ]
DEMENDOZA, D [1 ]
机构
[1] UNIV NACL ROSARIO,FAC CIENCIAS BIOQUIM & FARMACEUT,DEPT MICROBIOL,SUIPACHA 531,RA-2000 ROSARIO,ARGENTINA
关键词
D O I
10.1111/j.1365-2958.1993.tb01598.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacillus subtilis synthesizes, almost exclusively, saturated fatty acids, when grown at 37-degrees-C. When cultures were transferred from 37-degrees-C to 20-degrees-C, a chloramphenicol- and rifampicin-sensitive synthesis of a C-16 unsaturated fatty acid was observed. Synthesis of this compound reached a plateau after 5 h at 20-degrees-C, reaching levels of 20% of the total fatty acid content. [C-14]-labelled fatty acids attached as thioesters to acyl-carriers compounds, such as coenzyme A (CoA) or acyl-carrier protein (ACP) synthesized de novo by glycerol-requiring auxotrophs deprived of glycerol to arrest phospholipid synthesis, could not be desaturated at 20-degrees-C. Desaturation of these fatty acids was readily observed when glycerol was restored to the cultures allowing resumption of transfer of acyl-moieties from acyl-thioesters to phospholipid. It was also observed that depletion of the pools of CoA and ACP by starvation of pantothenate auxotrophs had no effect on the observed synthesis of unsaturated fatty acid at 20-degrees-C. The overall results indicate that synthesis of unsaturated fatty acids in B. subtilis is a cold-inducible process and that phospholipids are obligate intermediates in this fatty acid desaturation pathway.
引用
收藏
页码:535 / 542
页数:8
相关论文
共 24 条
  • [1] ISOLATION AND CHARACTERIZATION OF BACILLUS-SUBTILIS MUTANTS BLOCKED IN THE SYNTHESIS OF PANTOTHENIC-ACID
    BAIGORI, M
    GRAU, R
    MORBIDONI, HR
    DEMENDOZA, D
    [J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (13) : 4240 - 4242
  • [2] BLOCH K, 1970, ENZYMES, V5, P441
  • [3] CRONAN JE, 1975, J BIOL CHEM, V250, P5835
  • [4] Cronan Jr. J.E., 1987, ESCHERICHIA COLI SAL, P474
  • [5] OVERPRODUCTION OF CIS-VACCENIC ACID AND ALTERED TEMPERATURE CONTROL OF FATTY-ACID SYNTHESIS IN A MUTANT OF ESCHERICHIA-COLI
    DEMENDOZA, D
    GARWIN, JL
    CRONAN, JE
    [J]. JOURNAL OF BACTERIOLOGY, 1982, 151 (03) : 1608 - 1611
  • [6] THERMAL REGULATION OF MEMBRANE LIPID FLUIDITY IN BACTERIA
    DEMENDOZA, D
    CRONAN, JE
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1983, 8 (02) : 49 - 52
  • [7] DEMENDOZA D, 1983, J BIOL CHEM, V258, P2098
  • [8] DEMENDOZA D, 1993, IN PRESS BACILLUS SU
  • [9] DEMENDOZA D, 1988, PHYSL REGULATION MEM, V3, P119
  • [10] MUTANTS OF BACILLUS SPECIES ISOLATED ON THE BASIS OF PROTONOPHORE RESISTANCE ARE DEFICIENT IN FATTY-ACID DESATURASE ACTIVITY
    DUNKLEY, EA
    CLEJAN, S
    KRULWICH, TA
    [J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (24) : 7750 - 7755