NUCLEASE SENSITIVITY AND FUNCTIONAL-ANALYSIS OF A MAIZE HISTONE H3 GENE PROMOTER

被引：9

作者：

BRIGNON, P ^{[1
]}

LEPETIT, M ^{[1
]}

GIGOT, C ^{[1
]}

CHAUBET, N ^{[1
]}

机构：

[1] UNIV STRASBOURG 1, CNRS, INST BIOL MOLEC PLANTES, 12 RUE GEN ZIMMER, F-67070 STRASBOURG, FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 22卷 / 06期

关键词：

CHROMATIN; HISTONE PROMOTER; HYPERSENSITIVE SITES; NUCLEASE ACCESSIBILITY; NUCLEOSOMES; TRANSGENIC PLANTS;

D O I：

10.1007/BF00028973

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.

引用

页码：1007 / 1015

页数：9

共 25 条

[11] KAHL G, 1988, ARCHITECTURE EUKARYO, P301
[12] DNA-BINDING PROTEIN(S) INTERACTS WITH A CONSERVED NONAMERIC SEQUENCE IN THE UPSTREAM REGIONS OF WHEAT HISTONE GENES
KAWATA, T
NAKAYAMA, T
MIKAMI, K
TABATA, T
TAKASE, H
IWABUCHI, M
[J]. FEBS LETTERS, 1988, 239 (02) : 319 - 323
[13] CELL-SPECIFIC EXPRESSION OF PLANT HISTONE H2A GENES
KONING, AJ
TANIMOTO, EY
KIEHNE, K
ROST, T
COMAI, L
[J]. PLANT CELL, 1991, 3 (07) : 657 - 665
[14] A PLANT HISTONE GENE PROMOTER CAN DIRECT BOTH REPLICATION-DEPENDENT AND REPLICATION-INDEPENDENT GENE-EXPRESSION IN TRANSGENIC PLANTS
LEPETIT, M
EHLING, M
CHAUBET, N
GIGOT, C
[J]. MOLECULAR & GENERAL GENETICS, 1992, 231 (02): : 276 - 285
[15] LEPETIT M, 1991, PLANT CELL REP, V10, P401, DOI 10.1007/BF00232611
[16] HIGHLY CONSERVED HEXAMER, OCTAMER AND NONAMER MOTIFS ARE POSITIVE CIS-REGULATORY ELEMENTS OF THE WHEAT HISTONE-H3 GENE
NAKAYAMA, T
SAKAMOTO, A
YANG, P
MINAMI, M
FUJIMOTO, Y
ITO, T
IWABUCHI, M
[J]. FEBS LETTERS, 1992, 300 (02) : 167 - 170
[17] TISSUE-SPECIFIC EXPRESSION OF A WHEAT HIGH MOLECULAR-WEIGHT GLUTENIN GENE IN TRANSGENIC TOBACCO
ROBERT, LS
THOMPSON, RD
FLAVELL, RB
[J]. PLANT CELL, 1989, 1 (06) : 569 - 578
[18] Sambrook J., 1989, MOL CLONING LAB MANU
[19] ABSENCE OF STORAGE PROTEIN-SYNTHESIS IN THE EMBRYO OF ZEA-MAYS
SANCHEZMARTINEZ, D
GOMEZ, J
LUDEVID, MD
TORRENT, M
PUIGDOMENECH, P
PAGES, M
[J]. PLANT SCIENCE, 1987, 53 (03) : 215 - 221
[20] MULTILEVEL REGULATION OF REPLICATION-DEPENDENT HISTONE GENES
SCHUMPERLI, D
[J]. TRENDS IN GENETICS, 1988, 4 (07) : 187 - 191

← 1 2 3 →