ONLINE FULLY AUTOMATED-DETERMINATION OF CLOZAPINE AND DESMETHYLCLOZAPINE IN HUMAN SERUM BY SOLID-PHASE EXTRACTION ON EXCHANGEABLE CARTRIDGES AND LIQUID-CHROMATOGRAPHY USING A METHANOL BUFFER MOBILE-PHASE ON UNMODIFIED SILICA

被引：45

作者：

OLESEN, OV

POULSEN, B

机构：

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 622卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80247-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fully automated method for determination of clozapine and desmethylclozapine in human serum using high-performance liquid chromatography was developed. On-line solid-phase extraction was performed on an exchangeable cyanopropyl cartridge. The analytes were eluted with a methanol-ammonium acetate buffer mobile phase, separated on a silica column, and measured by ultraviolet detection at 261 nm. The total time for one analysis was 13 min. Inter-day variation was <6% and <8% for clozapine and desmethylclozapine, respectively. Detector response was linear in the range 30-3000 ng/ml. Comparison with liquid-liquid extraction showed good agreement. The patients had clozapine serum concentrations in the range 40-1500 ng/ml. Desmethylclozapine concentrations were 25% lower and closely related.

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页码：39 / 46

页数：8

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