GRAFTING OF GENETICALLY MODIFIED CELLS - EFFECTS OF ACETYLCHOLINE-RELEASE INVIVO

被引：9

作者：

MAYSINGER, D ^{[1
]}

PICCARDO, P ^{[1
]}

GOINY, M ^{[1
]}

CUELLO, AC ^{[1
]}

机构：

[1] KAROLINSKA INST, DEPT PHARMACOL, S-10401 STOCKHOLM 60, SWEDEN

来源：

NEUROCHEMISTRY INTERNATIONAL | 1992年 / 21卷 / 04期

关键词：

D O I：

10.1016/0197-0186(92)90086-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, microdialysis was used to investigate functional recovery of central cholinergic neurons in the forebrain of rats with cortical devascularizing lesions. Mature male rats were unilaterally lesioned by disruption of the pia arachnoid vessels and genetically modified fibroblasts secreting nerve growth factor (NGF) were placed at the site of the lesion. One month following surgery, microdialysis probes were installed in the remaining cortex and were perfused with artificial cerebrospinal fluid (csf) containing neostigmine (5 nM) and/or KCl (100 mM). The basal (non-stimulated) release of acetylcholine (ACh) in the cortex was similar in all experimental groups, whereas KCl stimulated release of ACh was significantly augmented (P < 0.05) in the ipsilateral remaining cortex in lesioned animals that have been implanted with fibroblasts secreting NGF. These results suggest that NGF secreted by genetically engineered fibroblasts modulates neuroplasticity in the adult mammalian CNS and may favour recovery of cortical function following injury.

引用

页码：543 / 548

页数：6

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