QUANTITATIVE-ANALYSIS OF PEPTIDES BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

被引：32

作者：

GUSEV, AI ^{[1
]}

WILKINSON, WR ^{[1
]}

PROCTOR, A ^{[1
]}

HERCULES, DM ^{[1
]}

机构：

[1] UNIV PITTSBURGH,DEPT CHEM,PITTSBURGH,PA 15260

来源：

APPLIED SPECTROSCOPY | 1993年 / 47卷 / 08期

关键词：

MASS SPECTROMETRY; LAMMA; MALDI; LASERS; PEPTIDES;

D O I：

10.1366/0003702934067847

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Quantitative analysis of peptides using internal standards was carried out with the use of matrix-assisted laser desorption/ionization (MALDI) on a time-of-flight mass spectrometer in a ferulic acid matrix. Spectra were collected and averaged over several areas of the sample surface at constant laser energy, and a defocused beam was used to improve reproducibility. A calibration curve was plotted, showing a correlation coefficient of 0.992 and a relative standard deviation of 1.83%. The accuracy and precision of this technique were sensitive to the crystal structure of the matrix and saturation effects of the instrument's detector.

引用

页码：1091 / 1092

页数：2

共 6 条

[1]

BAHR U, 1992, ANAL CHEM, V64, P2869

[2]

Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P436, DOI 10.1002/rcm.1290031208

[3] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS [J].