POTASSIUM-EVOKED AND CARBACHOL-EVOKED RELEASE OF [H-3] NORADRENALINE FROM HUMAN NEUROBLASTOMA-CELLS, SH-SY5Y

被引：74

作者：

MURPHY, NP ^{[1
]}

BALL, SG ^{[1
]}

VAUGHAN, PFT ^{[1
]}

机构：

[1] UNIV LEEDS, DEPT CARDIOVASC STUDIES, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 56卷 / 05期

关键词：

SH-SY5Y NEUROBLASTOMA CELLS; NORADRENALINE; RELEASE; MUSCARINIC RECEPTORS; CARBACHOL; DEPOLARIZATION;

D O I：

10.1111/j.1471-4159.1991.tb02085.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human neuroblastoma clone SH-SY5Y expresses potassium-, carbachol-, and calcium ionophore A23187-evoked, calcium-dependent release of [H-3]noradrenaline. Release in response to carbachol and potassium was greater than additive. Atropine (K(i) = 0.33 nM), hexahydrosiladifenidol (K(i) = 18 nM), and pirenzepine (K(i) = 1,183 nM) completely inhibited the carbachol-evoked noradrenaline release, an order of potency suggesting that an M3 receptor was linked to release. In contrast, noradrenaline release was only partially inhibited by the M2-selective antagonists methoctramine (10(-4) M) and AFDX-116 (10(-4) M), by approximately 14 and 46%, respectively. The nicotinic antagonist d-tubocurarine (10(-4) M) resulted in a partial inhibition of release, a finding suggesting that a nicotinic receptor may also be involved. SH-SY5Y provides a suitable cell line in which to study the biochemical mechanisms underlying the cholinergic receptor regulation of noradrenaline release.

引用

页码：1810 / 1815

页数：6