HIGH-VELOCITY MECHANICAL DNA TRANSFER OF THE CHLORAMPHENICOLACETYL TRANSFERASE GENE INTO RODENT LIVER, KIDNEY AND MAMMARY-GLAND CELLS IN ORGAN EXPLANTS AND INVIVO

被引：22

作者：

ZELENIN, AV ^{[1
]}

ALIMOV, AA ^{[1
]}

TITOMIROV, AV ^{[1
]}

KAZANSKY, AV ^{[1
]}

GORODETSKY, SI ^{[1
]}

KOLESNIKOV, VA ^{[1
]}

机构：

[1] NI VAVILOV GEN GENET RES INST,MOSCOW 117333,USSR

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

TRANSFECTION; HIGH-VELOCITY MICROPROJECTILE; CAT GENE; RODENT CELL INVITRO AND INVIVO;

D O I：

10.1016/0014-5793(91)80212-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p-chi-Casein-cat, p-beta-Casein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.

引用

页码：94 / 96

页数：3

共 19 条

[1] ALTERING THE GENOME BY HOMOLOGOUS RECOMBINATION [J].