PROTECTION BY ASCORBATE AGAINST ACETYLPHENYLHYDRAZINE-INDUCED HEINZ BODY FORMATION IN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENT ERYTHROCYTES

被引：52

作者：

WINTERBOURN, CC

机构：

[1] Department of Clinical Biochemistry, Christchurch Hospital, Christchurch

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1979年 / 41卷 / 02期

关键词：

D O I：

10.1111/j.1365-2141.1979.tb05853.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Summary. Ascorbate was found to inhibit oxidation of oxyhaemoglobin and Heinz body formation in glucose‐6‐phosphate dehydrogenase deficient red cells incubated with acetylphenylhydrazine. It is proposed that ascorbate can substitute for the glutathione which is depleted and act as a scavenger for drug free radicals generated during the reaction. Ascorbate was protective at concentrations only a little higher than that in normal blood, and the possibility that administration of ascorbate could protect GSH deficient cells against the action of oxidative drugs such as APH is considered. A simple method of quantitatively assessing Heinz body formation by measuring the turbidity of the lysed cells is described. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：245 / 252

页数：8

共 16 条

[11]

KOSOWER NS, 1974, GLUTATHIONE, P216

[12]

MILLS GC, 1957, J BIOL CHEM, V229, P189

[13] OXIDATION OF PHENYLHYDRAZINE - SUPEROXIDE AND MECHANISM [J].