ENHANCEMENT OF THE ENDOTHELIAL PRODUCTION OF PROSTACYCLIN BY INHIBITORS OF PROTEIN-SYNTHESIS

被引：2

作者：

BOEYNAEMS, JM

BOUTHERINFALSON, O

LAGNEAU, C

GALAND, N

机构：

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1990年 / 101卷 / 04期

关键词：

D O I：

10.1111/j.1476-5381.1990.tb14160.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Pretreatment of bovine aortic endothelial cells with cycloheximide enhanced their capacity to release prostacyclin in response to adenosine 5'-triphosphate (ATP) and bradykinin. 2. The action of cycloheximide was time-dependent; it became detectable after a 1 h exposure to the cells and was maximal after 3 h. 3. Puromycin mimicked the effect of cycloheximide. For these two agents, the enhancement of prostacyclin release was obtained at concentrations producing a partial inhibition of protein synthesis. 4. Cycloheximide increased the mobilization of free arachidonic acid induced by ATP in bovine aortic endothelial cells. 5. In conclusion, the synthesis of new proteins is not involved in the stimulatory action of ATP and bradykinin on prostacyclin production by bovine aortic endothelial cells. Despite the short half-life of prostaglandin H synthase in endothelial cells, cycloheximide and puromycin enhanced the release of prostacyclin induced by agonists. Our data suggest that this release might be under the control of rapidly turning-over phospholipase inhibitory proteins.

引用

页码：799 / 802

页数：4

共 21 条

[11]

FAGAN J.M., GOLDBERG A.L., Inhibitors of protein and RNA synthesis cause a rapid block in prostaglandin production at the prostaglandin synthase step, Proc. Natl. Acad. Sci. U.S.A., 83, pp. 2771-2775, (1986)

[12]

HULLIN F., RAYNAL P., RAGAB-THOMAS J.M.F., FAUVEL J., CHAP H., Effect of dexamethasone on prostaglandin synthesis and on lipocortin status in human endothelial cells, J. Biol. Chem, 264, pp. 3506-3513, (1989)

[13]

LAMBERT T.L., KENT R.S., WHORTON A.R., Bradykinin stimulation of inositol polyphosphate production in porcine aortic endothelial cells, J. Biol. Chem, 261, pp. 15288-15293, (1986)

[14]

MACLOUF J., A radioimmunoassay for 6‐keto‐PGF1α, Methods Enzymol, 86, pp. 273-286, (1981)

[15]

MORGAN-BOYD R., STEWART J.M., VAVREK R.J., HASSID A., Effects of bradykinin and angiotensin II on intracellular Ca++ dynamics in endothelial cells, Am. J. Physiol, 253, pp. C588-C598, (1987)

[16]

RAZ A., WYCKE A., NEEDLEMAN P., Temporal and pharmacological division of fibroblast cyclooxygenase expression into transcriptional and translational phases, Proc. Natl. Acad. Sci. U.S.A., 86, pp. 1657-1661, (1989)

[17]

SIEKEVITZ P., Uptake of radioactive alanine in vitro into the proteins of rat liver fractions, J. Biol. Chem, 195, pp. 549-565, (1952)

[18]

VAN COEVORDEN A., BOEYNAEMS K.M., Physiological concentrations of ADP stimulate the release of prostacyclin from bovine aortic endothelial cells, Prostaglandins, 27, pp. 615-626, (1984)

[19]

WICKRAMASINGHE J.A.F., MOROZOWICH W., HAMLIN W.E., SHAW S.R., Detection of PGF2α as pentafluorobenzyl ester by electron capture GLC., J. Pharm. Sci, 62, pp. 1428-1431, (1973)

[20]

WILLEMS C., DE GROOT P.G., POOL G.A., GONSALVEZ M.S., VAN AKEN W.G., VAN MOURIK J.A., Arachidonate metabolism in cultured human vascular endothelial cells. Evidence for two prostaglandin synthetic pathways sensitive to acetylsalicylic acid, Biochim. Biophys. Acta, 713, pp. 581-588, (1982)

← 1 2 3 →