LOCALIZATION OF THE MEMBRANE-BINDING SITES OF ERA IN ESCHERICHIA-COLI

被引：24

作者：

GOLLOP, N ^{[1
]}

MARCH, PE ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT BIOCHEM,675 HOES LANE,PISCATAWAY,NJ 08854

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 2-3期

关键词：

ERA; GTP-BINDING; PROTEIN; ESCHERICHIA-COLI; MEMBRANE; IMMUNOELECTRON MICROSCOPY;

D O I：

10.1016/0923-2508(91)90045-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Era is a GTP-binding protein that is essential for normal cell growth and division in Escherichia coli. In view of the fact that eukaryotic proteins similar to Era are membrane-associated and important in membrane signalling pathways, experiments were carried out to establish the intracellular location of Era. Immunoelectron microscopy was employed to demonstrate that Era resides at or very near the internal surface of the cytoplasmic membrane, which is a location expected for a membrane signalling protein. In addition, Era occurs in patches that may correspond to areas that are potential sites of septation.

引用

页码：301 / 307

页数：7