BIOCONVERSIONS IN AQUEOUS 2-PHASE SYSTEMS

被引:120
作者
ANDERSSON, E [1 ]
HAHNHAGERDAL, B [1 ]
机构
[1] UNIV LUND, CTR CHEM, DEPT APPL MICROBIOL, POB 124, S-22100 LUND, SWEDEN
关键词
Aqueous two-phase systems; bioconversions; enzymes; microbial cells;
D O I
10.1016/0141-0229(90)90095-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioconversions involving enzymes and/or microbial cells in aqueous two-phase systems are reviewed. The partitioning of biocatalysts, substrates, and products is discussed in relation to their size. The efficiency of retaining biocatalysts in aqueous two-phase systems is summarized in relation to other methods of recirculating. The influence of phase components on the activity and the stability of enzymatic biocatalysts is exemplified with penicillin acylase and the cellulolytic enzyme system, and the effect of phase components on biocatalytic living cells is exemplified with the production of α-amylase with Bacillus sp. Process design costs in bioconversions in aqueous two-phase systems are briefly summarized. © 1990.
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页码:242 / 254
页数:13
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