EFFLUX-MEDIATED ANTISEPTIC RESISTANCE GENE QACA FROM STAPHYLOCOCCUS-AUREUS - COMMON ANCESTRY WITH TETRACYCLINE-TRANSPORT AND SUGAR-TRANSPORT PROTEINS

被引:251
作者
ROUCH, DA [1 ]
CRAM, DS [1 ]
DIBERARDINO, D [1 ]
LITTLEJOHN, TG [1 ]
SKURRAY, RA [1 ]
机构
[1] MONASH UNIV, DEPT BIOCHEM, CLAYTON, VIC 3168, AUSTRALIA
关键词
D O I
10.1111/j.1365-2958.1990.tb00565.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Resistance to intercalating dyes (ethidium, acriflavine) and other organic cations, such as quaternary ammonium-type antiseptic compounds, mediated by the Staphylococcus aureus plasmid pSK1 is specified by an energy-dependent export mechanism encoded by the qacA gene. From nucleotide sequence analysis, qacA is predicted to encode a protein of M(r) 55017 containing 514 amino acids. The gene is likely to initiate with a CUG codon, and a 36bp palindrome immediately preceding qacA, along with an upstream reading frame with homology to the TetR repressors, may be components of a regulatory circuit. The putative polypeptide specified by qacA has properties typical of a cytoplasmic membrane protein, and is indicated to be a member of a transport protein family that includes proteins responsible for export-mediated resistance to tetracycline and methylenomycin, and uptake of sugars and quinate. The analysis suggests that N- and C-terminal regions of these proteins are involved in energy coupling (proton translocation) and substrate transport, respectively. The last common ancestor of the qacA and related tet (tetracycline resistance) lineages is inferred to have been repressor controlled, as occurs for modern tet determinants from Gram-negative, but not those from Gram-positive, bacteria.
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页码:2051 / 2062
页数:12
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  • [1] Ball P.R., Shales S.W., Chopra I., Plasmid‐mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic, Biochem Biophys Res Commun, 93, pp. 74-81, (1980)
  • [2] Barton J.E., Sternberg J.E., A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structures, J Mol Biol, 198, pp. 327-337, (1987)
  • [3] Beck C.F., Mutzel R., Barbe J., Muller W., A multifunctional gene (tetR) controls Tn 10‐encoded tetracycline resistance, J Bacteriol, 150, pp. 633-642, (1982)
  • [4] Bennetzen J.L., Hall B.D., The primary structure of the Saccharyomyces cerevisiae gene for alcohol dehydrogenase I, J Biol Chem, 257, pp. 3026-3031, (1982)
  • [5] Birnbaum M.J., Haspel H.C., Rosen O.M., Cloning and characterisation of a cDNA encoding the rat brain glucose‐transporter protein, Proc Natl Acad Sci USA, 83, pp. 5784-5788, (1986)
  • [6] Byeon W.H., Weisblum B., Post‐transcriptional regulation of chloramphenicol acetyl transferase, J Bacteriol, 158, pp. 543-550, (1984)
  • [7] Byrne M.E., Rouch D.A., Skurray R.A., Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamicin‐tobramycin‐kanamycin‐resistance transposon Tn4001., Gene, 81, pp. 361-367, (1989)
  • [8] Celenza J.L., Marshall-Carlson L., Carlson M., The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein, Proc Natl Acad Sci USA, 85, pp. 2130-2134, (1988)
  • [9] Chen C., Chin J.E., Kozumitsu U., Clark D.P., Pastan I., Gottesman M.M., Roninson I.B., Internal duplication and homology with bacterial transport proteins in the mdr1 (P‐glycoprotein) gene from multidrug‐resistant human cells, Cell, 47, pp. 381-389, (1986)
  • [10] Cheng Q., Michels C.A., The maltose permease encoded by the MAL61 gene of Saccharomyces cerevisiae exhibits both sequence and structural homology to other sugar transporters, Genetics, 123, pp. 477-484, (1989)