EFFLUX-MEDIATED ANTISEPTIC RESISTANCE GENE QACA FROM STAPHYLOCOCCUS-AUREUS - COMMON ANCESTRY WITH TETRACYCLINE-TRANSPORT AND SUGAR-TRANSPORT PROTEINS

被引：251

作者：

ROUCH, DA ^{[1
]}

CRAM, DS ^{[1
]}

DIBERARDINO, D ^{[1
]}

LITTLEJOHN, TG ^{[1
]}

SKURRAY, RA ^{[1
]}

机构：

[1] MONASH UNIV, DEPT BIOCHEM, CLAYTON, VIC 3168, AUSTRALIA

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 12期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00565.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Resistance to intercalating dyes (ethidium, acriflavine) and other organic cations, such as quaternary ammonium-type antiseptic compounds, mediated by the Staphylococcus aureus plasmid pSK1 is specified by an energy-dependent export mechanism encoded by the qacA gene. From nucleotide sequence analysis, qacA is predicted to encode a protein of M(r) 55017 containing 514 amino acids. The gene is likely to initiate with a CUG codon, and a 36bp palindrome immediately preceding qacA, along with an upstream reading frame with homology to the TetR repressors, may be components of a regulatory circuit. The putative polypeptide specified by qacA has properties typical of a cytoplasmic membrane protein, and is indicated to be a member of a transport protein family that includes proteins responsible for export-mediated resistance to tetracycline and methylenomycin, and uptake of sugars and quinate. The analysis suggests that N- and C-terminal regions of these proteins are involved in energy coupling (proton translocation) and substrate transport, respectively. The last common ancestor of the qacA and related tet (tetracycline resistance) lineages is inferred to have been repressor controlled, as occurs for modern tet determinants from Gram-negative, but not those from Gram-positive, bacteria.

引用

页码：2051 / 2062

页数：12

共 82 条

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