HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF INDIVIDUAL BILE-ACIDS IN SERUM FOR AUTOMATIC DIAGNOSIS OF VARIOUS LIVER AND BILIARY DISEASES

被引：8

作者：

ZHAO, RH

LI, BY

CHEN, N

ZHANG, YK

WANG, ZY

LU, PC

机构：

[1] CHINESE ACAD SCI,DALIAN INST CHEM PHYS,NATL CHROMATOG R&A CTR,DALIAN 116012,PEOPLES R CHINA

[2] NANTONG MED COLL,NANTONG,PEOPLES R CHINA

来源：

BIOMEDICAL CHROMATOGRAPHY | 1993年 / 7卷 / 03期

关键词：

D O I：

10.1002/bmc.1130070307

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.

引用

页码：139 / 142

页数：4

共 8 条

[1] PEAK IDENTIFICATION OF THE CONJUGATED BILE-ACIDS IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY BY USING THE CONJUGATION SELECTIVITY [J].

CHEN, N ;

ZHANG, Y ;

LU, P .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1992, 15 (09) :1523-1531

[2] A FULLY-AUTOMATIC METHOD FOR ANALYSIS OF INDIVIDUAL BILE-ACIDS IN THE SERUM USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR CLINICAL USE [J].

HASEGAWA, S ;

UENOYAMA, R ;

TAKEDA, F ;

CHUMA, J ;

SUZUKI, K ;

KAMIYAMA, F ;

YAMAZAKI, K ;

BABA, S .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1984, 7 (11) :2267-2287

[3]

KOWALSKI BR, 1972, J AM CHEM SOC, V4, P16

[4]

LI BY, 1989, CHIN J CHROMATOGR, V7, P165

[5]

STREET JM, 1989, BIOMED CHROMATOGR, V2, P229

[6] ANALYSIS OF CONJUGATED BILE-ACIDS IN HUMAN-SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING POST-COLUMN ENZYME REACTION AND OFF-LINE FLUORIMETRIC DETERMINATION [J].

SWOBODNIK, W ;

ZHANG, YY ;

KLUEPPELBERG, U ;

JANOWITZ, P ;

WECHSLER, JG ;

FUERST, P ;

DITSCHUNEIT, H .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 423 :75-84

[7]

VARMUZA K, 1980, PATTERN RECOGN, P64

[8] THE RETENTION BEHAVIOR OF CONJUGATED BILE-ACIDS IN REVERSED PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

ZHANG, YK ;

CHEN, N ;

ZAO, RH ;

LI, T ;

WANG, ZY ;

LU, PC .

BIOMEDICAL CHROMATOGRAPHY, 1992, 6 (05) :255-257

← 1 →