CONFORMATIONAL PROPERTIES OF A NOVEL MODIFIED NUCLEOSIDE, 5-FORMYLCYTIDINE, FOUND AT THE FIRST POSITION OF THE ANTICODON OF BOVINE MITOCHONDRIAL TRNA(MET)

被引：21

作者：

KAWAI, G

YOKOGAWA, T

NISHIKAWA, K

UEDA, T

HASHIZUME, T

MCCLOSKEY, JA

YOKOYAMA, S

WATANABE, K

机构：

[1] UNIV TOKYO,FAC ENGN,DEPT IND CHEM,BUNKYO KU,TOKYO 113,JAPAN

[2] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOL SCI,MIDORI KU,YOKOHAMA 227,JAPAN

[3] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

[4] UNIV TOKYO,GRAD SCH SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1994年 / 13卷 / 05期

关键词：

D O I：

10.1080/15257779408011889

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conformational properties of a novel modified nucleoside, 5-formylcytidine (f(5)C), which is found at the first position of the anticodon of bovine mitochondrial tRNA(Met), were analyzed by H-1-NMR spectroscopy. f(5)C has a normal amino tautomeric form at position 4 of the base moiety. The results indicate the presence of an intramolecular hydrogen bond between the carbonyl of the 5-formyl group and the 4-amino function. f(5)C was found to exhibit the C3'-endo conformation exclusively and the enthalpy difference (Delta H) between the C2'-endo and C3'-endo forms was found to be 1.56 +/- 0.13 kcal/mol, indicating f(5)C to be one of the most conformationally rigid nucleosides yet analyzed. The conformational rigidity of f(5)C may contribute to regulation of codon recognition by tRNA(Met).

引用

页码：1189 / 1199

页数：11

共 18 条

[1] CONFORMATIONAL-ANALYSIS OF SUGAR RING IN NUCLEOSIDES AND NUCLEOTIDES - IMPROVED METHOD FOR INTERPRETATION OF PROTON MAGNETIC-RESONANCE COUPLING-CONSTANTS [J].